Physiological levels of blood coagulation factors IX and X control coagulation kinetics in anin vitromodel of circulating tissue factor

Kinetics of the tissue factor-dependent activation of coagulation Factors IX and X in a bovine plasma system.

The kinetics of the tissue factor-dependent activation of Factors IX and X have been studied in a bovine plasma system. Heat-defibrinated plasma, which lacks Factors VIII, V, and fibrinogen, was depleted of Factors IX and X by using specific antibodies coupled to Sepharose. Activation initiated by the addition of bovine brain thromboplastin was studied by adding back to the plasma radiolabeled ...

Factor IX in intravascular and extravascular blood coagulation.

The tissue factor requirement in blood coagulation.

Formation of thrombin is triggered when membrane-localized tissue factor (TF) is exposed to blood. In closed models of this process, thrombin formation displays an initiation phase (low rates of thrombin production cause platelet activation and fibrinogen clotting), a propagation phase (>95% of thrombin production occurs), and a termination phase (prothrombin activation ceases and free thrombin...

Proteolytic inactivation of blood coagulation factor IX by thrombin.

Coagulation factor IX is a vitamin K-dependent glycoprotein that circulates in blood as a precursor of a serine protease. Incubation of human factor IX with human alpha-thrombin resulted in a time and enzyme concentration-dependent cleavage of factor IX yielding a molecule composed of a heavy chain (mol wt 50,000) and a doublet light chain (mol wt 10,000). The proteolysis of factor IX by thromb...

A therapeutic concentrate of coagulation factors II, IX and X from citrated, factor VIII-depleted plasma.

A simple procedure is described for t he large-scale absorption on t o DEAEcelluiose of coagulation factors II , I X and X from citrated, factor VHI-depleted plasma. The coagulation factors are elutcd frontally f rom the exchanger in a high yield and hi a form suitable f o r therapeutic use, without further fractionation. The lyophilised con­ centrate is very stable without the addition of hepa...